Standardization of the method to obtain therapeuticquality platelet-rich plasma

Abstract

Aurora Martínez-Romero*, José Luis Ortega-Sánchez4, Reyna Margarita HernándezRamos1, José Prospero Hernández-de-la-Fuente5, Maribel Cervantes-Flores2,NormaUrtiz-Estrada2, Estela Ruiz-Baca2and José de Jesús Alba-Romero1,

Platelet-rich plasma (PRP) is a reliable source for obtaining cells to regenerate tissues, with ease of availability inorder to implement and standardize the ideal methodology in centrifugation strength and time for obtaining therapeutic-quality PRP, allowing its application to provide better and rapid recovery of muscular injuries, tendinitis, bone and ligament lesions. To evaluate PRP therapy, 150 patients with muscular lesions, tendinitis, shoulder, knee, ankle, hand and elbow injuries were treated. On application of PRP, we obtained 100% clinically significant symptomatic improvement in all 150 patients treated, who had musculoskeletal and ligament injuries, with a marked reduction of pain and inflammation. We concluded that the ideal concentration for obtaining PRP is at 1000 rpm with a time of 5 min; in addition, under these conditions the plasma lacks leukocytes and erythrocytes. The results were reproducible because the experiment was repeated at two institutions under the same conditions and similar results were obtained. The regeneration obtained in the affected patients is due to the fact that growth factors were released from the activated platelets; these initiate and modulate cicatrization in the tissues, which is a recent innovation to promote cicatrization, accelerating the power of tissue regeneration, with a platelet concentrate suspended in plasma.