Molecular characterization of antigens extracted from hydatid cysts of human and other intermediate hosts

Abstract

E. Ihsan Al-Saimary1*, N. Maani Al-Shemari1and M. A. Mohammed Al-Fayadh2

This research is a study on the molecular level of Echinococcus granulosus (larval stage) on, where 7 samples of hydatid cysts were collected from parasite intermediate hosts: Human (liver, spleen, lung) and liver of sheep, goat, cattle and buffaloes. DNA was extracted from germinal layer cells of hydatid cysts which were isolated shortly or preserved for various periods in 70% ethanol. Genetic analysis of isolated DNA from hydatid cysts collected from human and animals was done by polymerase chain reaction (PCR) to determine genetic variation depending on random amplified polymorphic DNA. In the present study, 10 primers were used during which the genetic variations were revealed among isolated (extracted DNA) hydatid cysts which was collected from human and other intermediate hoists except cows and buffaloes. The results of this study showed the following: 1. One primer (OPA – 01) was able to diagnose sample 1 which represent the isolated DNA of liver hydatid cyst obtained from human at age group 10 – 20 years old: 2. The ability of primer OPC – 10 to determine fingerprinting of DNA sample of sheep liver hydatid cyst. 3. The ability of primer OPC – 05 to determine fingerprinting of DNA sample of human spleen hydatid cyst which was obtained from human at age group 30 – 40 years old. The ability of primer OPE – 07 to determine fingerprinting of DNA sample of Goat liver hydatid cyst; 5. Amplification process to the DNA samples extracted from cows and buffaloes liver hydatid cysts was not completed by using all 10 primers.

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