International Journal of Clinical Biochemistry received 17 citations as per Google Scholar report
D Jethva*, P Shruthi, S Patel, N Karbhari and N Savaliya
Magnesium estimation in serum is done by various methods like Arsenazo III, Ortho Vitros Microslide System, Atomaticabsortption, Calmagite, Xylidyl blue, Methylthymol blue. One of the methods is Xylidyl blue in which chelating agents EDTA or EGTA can be used. In this study, Comparison is done between EDTA and EGTA as chelating agent. EGTA is a chelating agent with a much higher affinity for Ca2+ at pH 11.00. EDTA is a chelating agent for di and trivalent metal ions. EDTA binds to metals via four carboxylate and two amine groups. Magnesium is abundant cation in the human body. Majority is present in bone and muscle, Extra Cellular Fluid (ECF). Magnesium is a responsible for energy metabolism and the synthesis of proteins and nucleic acids. Methods-Measurement of magnesium is done by Xylidyl blue method using EGTA and EDTA as Chelating agent.
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