Kangin Chung-hee Rema
The aim of this research was to study genetic polymorphism in growth hormone (GH) locus and its association with body weight of 43 Grati dairy cows ranging from 2 to 4 years old. Polymorphism of GH locus was ascribed using a PCR-RFLP method involving restricted enzyme Msp1 on 2% agarose gel. All data were subjeced to statistical analysis based on one way classification model using a statistical software package of Genstat 200 version 2. The results showed that frequencies of normal allel (Msp1+ ) and mutant allel (Msp- ) were 0.34 and 0.66 respectively with 0.22 polymorphism was found in this locus. There was significant relationship between Msp1+ /Msp1+ , Msp1+ /Msp1- and Msp1- /Msp1- genotypes and body weight (P < 0.05) but Msp1+ /Msp1+ and Msp1+ /Msp1- genotypes had a stronger correlation to the higher body weight than Msp1- /Msp1- genotype.
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