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RACHANA SRIPATHI
K JAGDISHKUMAR AND HS RAJANI
D NARAYANAPPA
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RACHANA SRIPATHI
K JAGDISHKUMAR AND HS RAJANI
D NARAYANAPPA
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Journal of Fever and Tropical Diseases

S H O R T C O M M U N I C A T I O N 

Comparative Study of Dot Enzyme Immunoassay (Typhidot-M) and Widal Test in the Diagnosis of Typhoid Fever 

D NARAYANAPPA, RACHANA SRIPATHI, K JAGDISHKUMAR AND HS RAJANI

Department of Pediatrics, JSS Medical College, Mysore, India.

Corresponding Author's E-mail: sinchabhi@yahoo.com

Abstract

We compared the sensitivity and specificity of Typhidot-M and Widal test with blood culture (gold standard) for diagnosing typhoid fever in 105 children aged 1-15 years admitted with clinical suspicion of typhoid fever. Of the 105 cases, blood culture was positive for S.typhi in 41 (39%) children, Widal test was positive in 48 (45.7%) and Typhidot-M was positive in 78 (74.3%) cases. Sensitivity and specificity of Typhidot-M was 92.6% and 37.5% while sensitivity and specificity of Widal test was 34.1% and 42.8%, respectively. In children with fever of less than 7 days duration, Typhidot-M was positive in 97%, compared to 24.2% by Widal test. Typhidot-M is a simple and sensitive test for early diagnosis of typhoid fever in children. 

Key words: Blood culture, Salmonella typhi, Typhoid fever, Typhidot-M, Widal test.