Hepatitis B virus genotypic patterns in Sudan reflects population movements

Vol. 3 (1), pp. 176-180, January, 2018

 

Full Length Research Paper

 

Hepatitis B virus genotypic patterns in Sudan reflects population movements

 

Eman Yasin Salih, Walaa Saeed Eltahir Saeed, Maria Mohamed Hamad Satti, Gawahir Abbas, Isam Adam Suleiman, Eltahir Awad Gasim Khalil

 

Accepted 26 November, 2017

Hepatitis B virus (HBV) genotypes have important impacts on the natural course of infection, prognosis and treatment outcomes. HBV genotypes A, D and E are predominant in various parts of Africa. Sudan occupies a central position in Africa with waves of migration from surrounding territories and continuous influx of different HBV genotypes. This study aimed to determine HBV genotypic patterns in Sudan as a reflection of population movements. In a prospective, cross-sectional, analytical study and following scientific and ethics approvals, five hundred and thirty five HBV-ELISA/PCR confirmed individuals were consented for participation. DNA was extracted using the guanidine technique. HBV strains were genotyped using type-specific primers in a nested and multiplex PCR technique. Five hundred sera were reactive in ELISA tests and had HBV DNA traces. Thirty five individuals were ELISA HBs Ag non-reactive, but showed HBV DNA traces [Occult hepatitis]. Samples were typed as: genotype A [85%], genotype A+E [10%], genotype D [3%] while recombination of A, D and E was seen in 2%. In conclusion, HBV genotypes in Sudan are a mixture of genotypes A, D and E separately or in recombination reflecting previous and ongoing migration waves from surrounding territories. Sequencing studies are needed for further HBV genotypes confirmation.

 Keywords: HBV infection, Genotypic patterns, recombinant genotypes, Sudan.