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Flaviane Gomes Pereira
Paulo Roberto Dias dos Santos
Maria Auxiliadora Coelho Kaplan
Maria Teresa Villela Romanos
Davyson de Lima Moreira
Elsie Franklin Guimarães-
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Flaviane Gomes Pereira
Paulo Roberto Dias dos Santos
Maria Auxiliadora Coelho Kaplan
Maria Teresa Villela Romanos
Davyson de Lima Moreira
Elsie Franklin Guimarães-
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Global Journal of Medicinal Plants Research

Full Length Research Paper 

Antiviral activity of the crude n-hexane extract from leaves of Piper lepturum var. angustifolium (C.DC.) Yunck. (Piperaceae)

Flaviane Gomes Pereira, Paulo Roberto Dias dos Santos, Elsie Franklin Guimarães-, Maria Teresa Villela Romanos, Maria Auxiliadora Coelho Kaplan and Davyson de Lima Moreira

1Instituto Federal de Educação, Ciência e Tecnologia do Rio de Janeiro, Rua Senador Furtado 121/125, Maracanã, Rio de Janeiro, RJ, CEP: 26530 – 060, Brazil. 

2Instituto de Pesquisas Jardim Botânico do Rio de Janeiro, Rua Pacheco Leão, 915, Rio de Janeiro, RJ, CEP: 22460 – 030, Brazil. 

3Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Góes, Departamento de Virologia, Avenida Carlos Chagas Filho, CCS, Bloco I, Cidade Universitária, Rio de Janeiro, RJ, CEP: 21941 – 590, Brazil. 

4Universidade Federal do Rio de Janeiro, Núcleo de Pesquisa de Produtos Naturais, Avenida Carlos Chagas Filho, 373 Edifício do Centro de Ciências da Saúde (CCS), Bloco H, Cidade Universitária. CEP:21941 – 902, Brazil. 

5Fundação Oswaldo Cruz, Produtos Naturais – Farmanguinhos, Instituto de Tecnologia em Fármacos, Rua Sizenando Nabuco 100, Manguinhos, Rio de Janeiro, RJ, Brasil.

*Corresponding author. E-mail: flaviane.gp@gmail.com 

Accepted 19 October, 2013 

Piper lepturum var. angustifolium (C.DC.) Yunck. belongs to the family Piperaceae which is widely found from North to South of Brazil. Crude n-hexane extract from leaves of P. lepturum var. angustifolium was assayed to anti-herpes simplex viruses (HSV) activity against HSV-1 and HSV-2 in cell cultures, incubated at 37°C for 48 h in a 5% CO2 atmosphere. Chemical profile of the extract was performed in a Shimadzu high performance liquid chromatography-diode array detector-ultraviolet (HPLC-DAD-UV), using a RP-18 column and mobile phase in gradient of acetonitrile and acid deionized water (pH = 3.0), at a flow rate of 1.0 ml/min. The HPLC analyses showed signals characteristics of non-polar compounds, mainly distributed between 50 and 70 min. UV spectra of the three main compounds (50.3% of the mixture) suggested isomers, with only one ʎmax at 235 nm. The crude n-hexanic extract showed 94.4% inhibition to HSV-1 with ED50 value of 5.2 µg/ml and selective index superior to 38.4. Considering HSV-2, the extract promoted 92.7% of inhibition with ED50 value of 1.1 µg/ml and selectivity index (SI) superior to 181.8. These results point out this species of Piperaceae as a source of active compounds for the treatment of infections caused by herpes simplex virus. 

Key words: Antiviral activity, herpes simplex virus type 1, herpes simplex virus type 2, Piper lepturum var. angustifolium, Piperaceae, high performance liquid chromatography-diode array detector-ultraviolet (HPLC-DAD-UV).