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Magdi Boutros
Ahmed Ghali
Farid Fattah
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Magdi Boutros
Ahmed Ghali
Farid Fattah
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Global Journal of Medicinal Plants Research

ISSN: 0274-3248 Vol. 2 (1), pp. 105-116, January, 2016.                                                              

 

Full Length Research Paper

 

Isolation of major compounds from the bioactive fraction as well as their anti-inflammatory activities

 

 

 Magdi Boutros*, Ahmed Ghali and Farid Fattah

 

 Accepted 29 December, 2014

A bio-guided fractionation of the defatted ethanolic extract (DEE) of the aerial parts of Trachelospermum jasminoides and its fractions: ethyl acetate fraction (EAF), chloroform fraction (CF) and n-butanol fraction (BF) were carried out, using carragenan induced rat paw edema method, to evaluate the in vivo acute anti-inflammatory potential at a given dose of 100 mg/kg body weight compared to indomethacin (20 mg/kg body weight). The EAF revealed the highest anti-inflammatory activity (76.92%) relative to the DEE and the CF (63.82 and 48.75%, respectively) at the same tested dose, while the BF was significantly inactive. The EAF and its major isolated compounds were investigated to determine the level of pro-inflammatory cytokines using enzyme-linked immunosorbant assay (ELISA). Seven major compounds were isolated from the EAF, identified as: trachelogenin, nor-trachelogenin and tracheloside, in addition to apigenin, luteolin, quercetin and luteolin-7-O-β-D-glucopyranoside. Identification of the isolated compounds was achieved by their physico-chemical properties and spectral analysis (1D and 2D NMR). The EAF and the isolated compounds inhibited the excessive production of tumor necrosis factor-alpha (TNF-α) and interleukin (IL)-1β. Furthermore, the liquid chromatography/electrospray ionization-tandem mass spectrometry (LC/ESI-MS) for the bioactive EAF was carried out to complete the phytochemical picture. The results of this study verified that the EAF and the major compounds exert their action through inhibition of TNF-α and IL-1 β.

Key words: Trachelospermum jasminoides, tumor necrosis factor-alpha (TNF-α), interleukin (IL)-1β, liquid chromatography/electrospray ionization-tandem mass spectrometry (LC/ESI-MS). 

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