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Fawzi Alsheyab
Nizar Abuharfil
Dalal Awad
Mohanad Aldarouish
Yousef Mosameh
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Fawzi Alsheyab
Nizar Abuharfil
Dalal Awad
Mohanad Aldarouish
Yousef Mosameh
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Global Journal of Molecular Evolution and Genomic

Full Length Research Paper 

Preparation and partial characterization of monoclonal antibody against human phosphdiesterase 5 (PDE5)

Fawzi Alsheyab, Nizar Abuharfil, Mohanad Aldarouish, Dalal Awad and Yousef Mosameh 

1Department of Biotechnology, Jordan University of Science and Technology (JUST), Irbid- Jordan.

2Department of Biological Sciences, Yarmouk University, Irbid-Jordan.

3Renad Medical Laboratory, Amman-Jordan.

*Corresponding author. E-mail: ymosameh@hotmail.com. Tel: +962 777665780 

Accepted 1 June, 2013

Phosphodiesterase 5 (PDE5) is a catalytic enzyme for degradation of cyclic guanosine monophosphate (cGMP) in human smooth muscle cells. Inhibition of this enzyme by certain chemicals including sildenafil, vardenafil and tadalafil, is considered a breakthrough treatment of erectile dysfunction. Unfortunately, these drugs are associated with several side effects. In this study, another strategy is adopted in the inhibition of the PDE5 enzyme by using a monoclonal antibody (Mab) against the enzyme. The Mab was prepared by immunizing a mouse, taking the spleen to make single cell suspension of lymphocytes. Hybridoma cells were obtained by fusion of BALB/c myeloma cells with the immunized lymphocytes using the polyethylene glycol method. Several clones were produced that have been separated by limiting dilution method. The antibodies were tested by enzyme-linked immunosorbent assay (ELISA) and western blot. The obtained Mab has titer of 625, immunoglobulin G1 (IgG1) isotype and 3.9 × 104 mol-1 affinity constant. 

Key words: Phosphodiesterase (PDE), chemical inhibition, monoclonal antibody (Mab).