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Piyada Theerakulpisut
AL-Jewari Hazim
Al-Hadeethy Muazaz
BarusruxSahapat BarusruxSahapat
AL-Mshhdani Athiya
Jawad Mohammed M
AL-Khesraji Talib
Nantawan Kanawapee
Authors Articles on Pubmed
Piyada Theerakulpisut
AL-Jewari Hazim
Al-Hadeethy Muazaz
BarusruxSahapat BarusruxSahapat
AL-Mshhdani Athiya
Jawad Mohammed M
AL-Khesraji Talib
Nantawan Kanawapee
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Global Journal of Molecular Evolution and Genomic

Full Length Research Paper

Genetic Diversity and Relationships among Verbascum Species in Iraq by RAPD-PCR Technique

Al-Hadeethy Muazaz, Jawad Mohammed M, Nantawan Kanawapee, AL-Jewari Hazim, AL-Mshhdani Athiya, AL-Khesraji Talib, Barusrux Sahapat and Piyada Theerakulpisut

1Department of Biology, College of Education - Ebn Al- Haitham, University of Baghdad, Baghdad, Iraq.

2Applied Taxonomic Research Center, Department of Biology, Faculty of Science.KhonKaen University, Thailand

3Department of Biology, College of Education, University of Tikrit, Tikrit, Iraq.

4Centre for Research and Development of Medical Diagnostic Laboratories (CMDL), Faculty of Associated Medical Sciences, KhonKaen University, KhonKaen, 40002, Thailand.

Corresponding author Email: lubni_a75@yahoo.com

Accepted 27th March, 2014

This study was aimed to determine the genetic diversity and relationships among 20 local Verbascum species by using the Random Amplified Polymorphic DNA (RAPD) technique. To achieve this, Total genomic DNA of Verbascum plant was extracted from leaves using two different methods, by CTAB method using 2-3 g of leaves, with purity of 1.5-1.9, and also by using commercial kit, PCR reactions were carried out using 17 random decamere-nucleotide primers. Percentage of monomorphic and polymorphic bands varies for each primer used with each species and the lowest numbers of polymorphic bands were 2 with OPX16, which showed the polymorphism (50%), produced 4 bands which only 2 were polymorphic. OPA5 and OPW13 showed the highest number of bands (9) and all are polymorphic (100%). From total of 119 alleles that detected, 103 were polymorphic (86.55%) and 16 monomorphic Moreover. A total of 119 polymorphic bands were scored and used for the analysis of genetic distances. Band sizes varied between 100 and 1900 bp. According to the results of genetic distance and relationships illustrated, the ability to resolve genetic variation among different Verbascum species may relate to the number of polymorphic bands detected with marker technique. Dendrogram was constructed using UPGMA cluster analysis and depicted genetic relationships among 20Verbascum species, showed the 2 major clusters depending on their ancestor and their morphological traits. The information generated from this study can be used in the future for Verbascum breeding and improvement programs.

Keywords: Molecular analysis, Verbascum, RAPD markers, Iraq, phylogenetic tree