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Nour Eissa
Hong Yao
Hiam Elabd
Han-Ping Wang
Adel Shaheen
Vikas Kumar
Amany Abbass
Zhi-Gang Shen
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Nour Eissa
Hong Yao
Hiam Elabd
Han-Ping Wang
Adel Shaheen
Vikas Kumar
Amany Abbass
Zhi-Gang Shen
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Global Journal of Fisheries and Aquaculture

ISSN:  2408-5464 Vol. 3 (6), pp. 247-256, August, 2015

Full Length Research Paper

Stress, immune and growth responses of bluegill sunfish (Lepomis macrochirus) to different environmental temperatures as referred by related gene expression 

Hiam Elabd1,2, Vikas Kumar1, Nour Eissa1, Zhi-Gang Shen1, Hong Yao1, Adel Shaheen2, Amany Abbass2 and Han-Ping Wang1* 

1Aquaculture Genetics and Breeding Laboratory, the Ohio State University South Centers, Piketon, Ohio, 45661, USA.

2Department of Fish Diseases and Management, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh, 13736, Egypt.

*Corresponding author. E-mail: hiam_abd@yahoo.com

Accepted 14 August, 2015

Abstract

Global temperature climate changes affect stress, immune function and growth of aquatic animals. The aim of this study was to investigate the effect of different temperatures on oxidative stress, thermal stress, immune response and growth in bluegill sunfish (Lepomis macrochirus). The experiment included four groups that were exposed to different water temperatures: 10ºC (10.2 ± 0.05), 20ºC (20 ± 0.06), 30ºC (29.7 ± 0.09), and 35ºC (34.7 ± 0.05) for six weeks. Fish were sampled at day 0, 3 weeks, and 6 weeks. Significantly higher (P < 0.05) erythrocyte lysate superoxide dismutase (SOD) wasobserved in the 10ºC, 30ºC and 35ºC groups at day 0 and 3 week, but more pronounced at the 6th week. Heat Shock protein90 (HSP-90) was significantly up-regulated at higher temperatures (20, 30, and 35ºC), while down-regulated at 10ºC. Similarly, immune response biomarker α-2-macroglobulin (A2M) was also up-regulated in 20 and 30ºC groups whereas down-regulated in 10ºC group. All groups exhibited 100% survival except the 35ºC group (86% survival). The highest growth performance was observed for the 30ºC group and the lowest for the 10ºC group. Conclusively, findings support that SOD, HSP-90 and A2M could be considered as biomarkers for climate change and environmental monitoring as referred in fish species.

Key words: Bluegill, growth, Immune response, molecular biomarkers, oxidative stress, temperature, thermal stress