Acclimatization of micropropagated Musa cavendischii cultivar roatan plants submitted to doses of fertigation and auxin

Full Length Research Paper 

Acclimatization of micropropagated Musa cavendischii cultivar roatan plants submitted to doses of fertigation and auxin 

MM Elisama, EV Raymundo, VV Arturo, CÁG Virginia and CSJ Luis 

1Instituto Tecnológico del Valle de Oaxaca. Xoxocotlán, C.P. 71230, Oaxaca, México.

2CIIDIR- Instituto Politécnico Nacional, Unidad Oaxaca. Hornos # 1003, Santa Cruz Xoxocotlán, Oaxaca, Mexico. 

*Corresponding author. E-mail: jenriquezdelvalle@yahoo.com

Accepted 30 October, 2013 

Micropropagation of banana is suggested to produce great number of healthy plants but the success depends on the ability to transfer plants from in vitro conditions to greenhouse or field environment, at low cost and with high survival rates. In this study, the effects of the fertilization and indolebutyric acid (IBA) applied in the nutritive solution on growth of Musa cavendischii plantlets were evaluated during acclimatization process in greenhouse. First, in vitro plantlets of M. cavendischii were transplanted to pots of 150 cm3 containing perlite and transferred to greenhouse for their acclimatization during 11 weeks. The experiment was established according to a completely randomized design with 5 treatments and 20 replications per treatment. The experimental unit was one plant transplanted in each pot, resulting from the combinations of daily applications of 10 ml of the Steiner’s nutritive solution at 10, 25, 50, 75 and 100%, respectively (Factor A), without and with 1 mgL-1 of the auxin IBA (Factor B). After 11 weeks of acclimatization, the results shows that, the higher plants with respect to plant fresh, dry weight and height, and leaf width corresponded to the treatments from 75 to 100% of the Steiner’s solution. The IBA application had no significant effects on the growth of the M. cavendischii plants. There was no significant interaction between fertigation and IBA applications. The plants fertigated at 100% of nutriments concentration had 27.7 cm of height, 259.9 cm2 of foliar area, and 1.01 mg of chlorophyll g-1 of foliar fresh weight.

Key words: Acclimatization, fertilizer, micropropagation, indolebutyric acid, plant nutrition.