Phylogenomic analysis of mitochondrial DNA in the Huong pig: an indigenous pig of Vietnam

ISSN: 2408-5502 Vol. 5 (4), pp. 389-396, July, 2017

 

Full Length Research Paper

 

Phylogenomic analysis of mitochondrial DNA in the Huong pig: an indigenous pig of Vietnam

 

Thuy Thi Bich Vo, Hieu Duc Nguyen, Tuan Anh Bui, Minh Ngoc Nghiem, Eui Bae Jeung

 

Accepted 15 July, 2017

The Huong pig (Sus scrofa) has been included in a project aimed at preserving indigenous Vietnamese pig breeds. This study sequenced the complete mitochondrial (mt) genome of the indigenous Huong pig and determined its phylogenetic relationships with other Asian and European pig breeds. A complete mtDNA sequence of approximately 16,711 base pairs (bp) was obtained and submitted to GenBank (KY964306). In that mtDNA, the content levels of A, C, G, and T were 34.65%, 26.22%, 13.352%, and 25.78%, respectively. The whole mtDNA consisted of 37 genes, including 13 genes coding for proteins, 2 ribosomal RNA genes, 22 genes for tRNA, and a non-control region (D-loop). The length of the D-loop region was 1,315 bp and there were 27 repeat sequences (5’-tacacgtgcg). The phylogenetic relationships, based on both the mtDNA and the D-loop region, indicated the shortest genetic distance was between the Huong and Lantang pig breeds with close relationships to other Asian pig breeds such as South China, Yangtze the River, and Yellow River Region pig groups. In conclusion, the obtained Huong pig mtDNA sequence data can contribute to elucidating the relationships among Vietnamese indigenous pig breeds and support the selection of suitable livestock for pig breeding in the area.

Keywords: Mitochondrial genome, genetic distance, Huong pig, phylogenetic relationship